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Nanogold Labeling of Fibrils

Methodology Paper

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I developed a protocol to conjugate α-synuclein fibrils with nanogold particles, allowing their visualization by electron microscopy without reliance on antibodies. This method provided direct ultrastructural evidence of fibril location and enabled time-resolved tracking of their movement through cellular compartments. Through studying the biochemical properties of α-synuclein fibrils, I was able to devise a protocol that does not promote α-synuclein aggregation, keeping the α-synuclein suitable for internalization. 

protocol to conjugate α-synuclein fibrils with nanogold particles
Representative electron microscopy images of fibril α-synuclein morphology. Sonication allows for faster internalization and mimics protofibril species thought to seed aggregation in PD.

Representative electron microscopy images of fibril α-synuclein morphology. Sonication allows for faster internalization and mimics protofibril species thought to seed aggregation in Parkinson's disease.

Electron microscopy of nanogold-conjugated α-synuclein fibrils (sonicated) shows short preformed species (<100 nm) decorated with sparse nanogold beads. This confirms their structural integrity and suitability for trafficking studies.

Electron microscopy of nanogold-conjugated α-synuclein fibrils (sonicated) shows short preformed species (<100 nm) decorated with sparse nanogold beads. This confirms their structural integrity and suitability for trafficking studies.

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