Genetic & Molecular Biology
I apply a broad range of biochemical methods to probe protein trafficking, organelle stress, and cellular quality control. My expertise spans Western blotting, ELISA, PCR/qPCR, immunoprecipitation (including Lyso-IP), transfection, viral transduction, and lentiviral generation, combined with enzyme activity assays, protein purification, and reporter systems.
Other Biochemistry Skills
ELISA
Detection of cytokines, lysosomal proteins, and disease biomarkers using ELISA kits.
PCR and qPCR
amplification and quantification of target genes to measure editing and pathway activity.
Enzyme Activity Assays
cathepsin activity, GBA function, mitochondrial enzymes, and metabolic readouts.
Protein Purification and Characterization
isolation and validation of proteins for biochemical assays.