Microscopy
I use advanced microscopy—from live-cell confocal to super-resolution and electron microscopy—to uncover mechanisms in neurodegeneration and infection. These approaches reveal α-synuclein uptake, lysosomal stress, mitochondrial quality control, signaling, and protein trafficking. By integrating high-resolution imaging with quantitative pipelines, I connect cellular dynamics to mechanistic insights driving disease.
Use the links below to navigate to specific microscopy pages:
Other Imaging Skills
Multiplexed Imaging and Co-culture Assays
By combining fluorescent labeling with co-culture systems, I visualized neuron–glia interactions at single-cell resolution. In mixed cultures, activated microglia released cytokines that induced inclusion formation in adjacent neurons, whereas astrocytes suppressed pathology. Multiplexed imaging allowed simultaneous visualization of neurons, fibrils, and glia, uncovering how cell-cell communication shapes disease outcomes.
High-Content and Automated Imaging
I employed high-content confocal platforms to screen pharmacological and genetic perturbations in iPSC-derived neurons. This approach generated large-scale datasets of mitochondrial morphology, lysosomal dynamics, and α-synuclein inclusion burden. Coupled with automated pipelines (AutoMorphoTrack), these imaging experiments yielded reproducible, high-throughput readouts linking cellular phenotypes to disease mechanisms.