TRANSLATIONAL ASSAY SCIENCE

Proximity & Molecular Assay Development

A comprehensive overview of hands-on expertise in proximity-based assays, DNA-conjugated antibody platforms, PCR/qPCR optimization, flow cytometry, NGS, and cell-based assay development—spanning 10+ years across Harvard Medical School, McGill Neurological Institute, and biopharma collaborations.

ASSAY DEVELOPMENT

Assay Development & Optimization

10+ years developing and optimizing cell-based assays across high-throughput formats. Expertise in high-content and high-throughput assay development in 96- and 384-well formats. Assay miniaturization from benchtop to production scale with integration of orthogonal readouts including RT-qPCR, Western blot, ELISA/MSD/Luminex, and flow cytometry. Fit-for-purpose assay strategy with translational endpoint alignment, Z-factor optimization, and assay robustness metrics. SOP authoring and QC gate establishment for reproducible, scalable workflows.

10+

Years Hands-On Experience

96/384

Well Plate Formats

Biopharma Collaborations

20+

Peer-Reviewed Publications

ASSAY PLATFORM EXPERIENCE

Years of hands-on experience and proficiency across major immunoassay platforms

PCR & qPCR

PCR, qPCR & Nucleic Acid Analysis

Extensive PCR expertise spanning 10+ years: RT-qPCR as primary RNA validation tool, ddPCR for absolute quantification, primer design and optimization, nested PCR for cloning verification, TaqMan probe design and melt curve analysis, multiplex PCR optimization, and qPCR-based knockdown assessment in siRNA/shRNA workflows. Multi-gene normalization strategies, Tm balancing, and PCR additive optimization for robust, reproducible results. Applied across discovery, validation, and translational assay contexts.

10+

Years PCR/qPCR Experience

500+

Primer Pairs Designed

PCR Platforms Mastered

<2%

Typical Inter-Assay CV

PCR & qPCR COMPETENCY PROFILE

Proficiency across core PCR and qPCR technical competencies (0-10 scale)

ANTIBODY ASSAYS

Antibody-Based & Proximity Assays

Expert-level proficiency across 6+ immunoassay platforms: MSD (multiplex and singleplex electrochemiluminescence), Luminex multiplexed bead-based assays, traditional sandwich ELISA, Quanterix ultra-sensitive biomarker readouts, AlphaLISA label-free binding detection, and immunoprecipitation (IP, Co-IP, IP-MS). Experience with antibody pair optimization, dynamic range characterization, and lower limits of detection. Pharmacodynamic biomarker profiling, pathway activation readouts (phosphorylation status), inflammatory marker quantification, and target engagement validation across 50+ multiplexed biomarker panels and 1,200+ citations.

Immunoassay Platforms

50+

Multiplexed Biomarker Panels

1,200+

Total Citations

Sample Type Categories

BIOMARKER DETECTION PLATFORMS

Analyte profiling across major immunoassay platforms

FLOW CYTOMETRY

Flow Cytometry & Cell Sorting

5+ years of continuous FACS-based experience: FACS-based expression confirmation and immunophenotyping, cell sorting and population analysis on FACSymphony platform, binding assays, cell line generation with selection/validation via flow, apoptosis and cell viability assessment, multi-parametric gating and population definition. iPSC differentiation confirmation, transient and stable transfection screening, ligand-binding assays with typical sort purity exceeding 95%.

Years FACS Experience

12+

Marker Panels

Instrument Platforms

95%+

Typical Sort Purity

FLOW CYTOMETRY APPLICATION DISTRIBUTION

Primary use cases across 5+ years of FACS work

NGS & GENOMICS

Next-Generation Sequencing & Genomics

RNA-seq execution and project coordination spanning 30+ processed libraries. Library prep, bulk and single-cell RNA-seq (10x Genomics), scRNA-seq of iPSC-derived cultures, and CRO management (Novogene). Pathway-level interpretation, cross-model comparison (human iPSC vs rodent), and CRISPR knockout/siRNA knockdown validation via RNA-seq. Bioinformatics integration with transcriptomics, functional genomics, and multi-omics data fusion for comprehensive pathway mapping.

30+

RNA-seq Libraries Processed

Sequencing Modalities (Bulk + scRNA-seq)

3,420

DE Genes Identified (typical dataset)

Bioinformatics Pipelines

CELL CULTURE

Cell Culture & Tissue Engineering

10+ years experience across diverse cell culture systems. Human iPSC-derived neurons (dopaminergic), cardiomyocytes, renal, endodermal lineages. Mammalian lines including CHO, HEK293, Vero E6 (suspension and adherent) for pseudovirus assays. Primary cells including rodent neurons, glia, human astrocytes, and PBMCs. 2D and 3D organoid culture, single-use bioreactor operation at 5L scale, stable cell line generation (pool-to-clone funnel), and BSL-2/BSL-2+ compliance with enhanced protocols for Vero E6 pseudovirus work and iPSC-derived pathogenic systems.

10+

Years Cell Culture

15+

Cell Types Maintained

Bioreactor Scale

iPSC Differentiation Protocols

End-to-End Assay Development Workflow

Target Identification

Literature review, pathway analysis, biomarker selection

Assay Design

Platform selection, antibody pairing, primer/probe design

Optimization

Dose-response, time-course, Z-factor, QC gates

Validation

Orthogonal readouts, reproducibility, acceptance criteria

Scale-Up

SOP documentation, technology transfer, GMP alignment

DATA ANALYSIS

Data Analysis, Bioinformatics & Coding

Python and R custom packages for image analysis and quantification. Multi-parametric computational image analysis, QC automation, pathway-level transcriptomics interpretation, and high-throughput image segmentation. JMP for statistical QC, FlowJo for FACS data analysis, Benchling/ELN systems for laboratory informatics. Proficiency with 1,000+ analyzed datasets and 3× throughput improvement via automation.

Primary Languages (Python, R)

Analysis Platforms

1,000+

Datasets Analyzed

3×

Throughput Improvement via Automation

COMPUTATIONAL TOOLS PROFICIENCY

Expertise across core computational and analytical tools (0-10 scale)

COMMUNICATION

Scientific Communication & Collaboration

20+ peer-reviewed publications with 1,200+ citations, including first-author publications in Nature Neuroscience and Cell Reports. Cross-functional presentations, manuscript preparation, grant proposal writing. Biopharma partner briefings with GSK, Thermo Fisher, Nysnobio, Cajal, SGC, and Abcam. Training and mentoring of 10+ junior scientists in assay development, data analysis, and translational research methodologies.

20+

Publications

1,200+

Citations

Industry Partners

10+

Scientists Mentored

OPERATIONS

Laboratory Operations & Compliance

GMP/GLP/GxP compliance, SOP development and enforcement, ELN documentation (Benchling), regulatory-facing documentation, audit-ready reporting, BSL-2/BSL-2+ compliance with enhanced biosafety protocols for viral work including SARS-CoV-2 pseudovirus handling and iPSC-derived culture systems, equipment maintenance, reagent preparation and inventory management, and chain-of-custody documentation for precious samples. Quality-by-design principles, process validation, and continuous improvement methodologies for reproducible, compliant operations.