Interactive visualizations of CRISPR-based functional screening campaigns spanning mitophagy pathway discovery in iPSC-derived neurons, α-synuclein fibril uptake mechanism dissection, and SARS-CoV-2 viral entry characterization — from genome-wide pooled screens to arrayed target validation.
Nature · Under Review
Genome-wide CRISPR knockout screen in iPSC-derived dopaminergic neurons identifying regulators of PARK2-dependent mitophagy and neuroprotective targets. Integrated multiomics, quantitative imaging, and LC-MS/MS PK to demonstrate small-molecule neuroprotection in iPSC-derived and in vivo Parkinson’s disease models.
Each dot represents a gene. X-axis: log₂ fold-change of mitophagy flux; Y-axis: −log₁₀(p-value). Hover over significant hits for gene details. Dashed line = FDR < 0.05 threshold.
Ranked by phenotype Z-score from the primary pooled screen in iPSC-derived dopaminergic neurons.
Neuroprotection of iPSC-derived dopaminergic neurons treated with small-molecule Parkin agonist (compound ABY-101). IC₅₀ determined by mitochondrial membrane potential rescue.
Cell Reports · 2022 · 50+ Citations
Arrayed CRISPR screen in RPE1 cells identifying RAC1-dependent macropinocytosis as the major α-synuclein preformed fibril internalization pathway. RAC1 knockout reduced internalization, characterized by quantitative confocal and electron microscopy.
Ranked effect sizes for top endocytic regulators. Negative values indicate reduced α-synuclein PFF uptake upon gene knockout.
Relative contribution of each endocytic mechanism to α-synuclein fibril internalization, quantified by CRISPR KO effect sizes.
J. Biol. Chem. · 2021 · 500+ Citations
siRNA screen across endocytic machinery genes defining clathrin-mediated endocytosis as the primary SARS-CoV-2 cellular entry route. Established using pseudotyped lentivirus infectivity assays, siRNA knockdown of clathrin heavy chain, and quantitative confocal imaging across four cell lines.
GFP⁺ cell quantification (Opera Phenix) across four cell lines after siRNA knockdown of endocytic regulators.
Time-course of pseudovirus internalization with Pitstop-2 (clathrin inhibitor) vs. filipin (caveolin inhibitor) vs. DMSO control.
eLife · 2025
Multi-screen validation heatmap and single-cell transcriptomic embedding of CRISPR-perturbed iPSC-derived neurons. Integrates AutoMorphoTrack quantitative imaging with Seahorse XF metabolic flux analysis across all three screening campaigns.
Z-score normalized phenotypic effects of top hits across three independent CRISPR campaigns (mitophagy, α-syn uptake, viral entry). Rows = genes, columns = assay readouts.
Single-cell transcriptome embedding of iPSC-derived neurons after CRISPR KO of top mitophagy regulators. Colors indicate perturbation identity.
Oxygen consumption rate (OCR) in iPSC-derived neurons following CRISPR KO of PINK1, PARK2, BNIP3L, and FUNDC1 vs. non-targeting control.